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You are here:Home » Antibodies » Antibodies-Transcription Factors » Anti -Akt1, phosphorylated (Ser473) (Rac PKa, PKBa)

Anti -Akt1, phosphorylated (Ser473) (Rac PKa, PKBa)


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Clone Host Grade Applications
Monoclonal Rabbit Affinity Purified B
Catalog #A1125-03
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 0.07-0.4ug/ml of this lot detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 50ng/ml PDGF for 20 minutes.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5H45
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa. The immunizing
sequence is identical in mouse and bovine Akt1. Akt2 and Akt3 share significant homology with the peptide immunogen sequence.
SpecificityRecognizes phosphorylated Akt1/PKBa, Mr ~60kD. Crossreactivity to phosphorylated Akt2 and Akt3 likely based on sequence homology. Species Crossreactivity: Human and mouse. Predicted crossreactivity with rat, bovine, chicken, and Xenopus based on immunogen sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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