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You are here:Home » Antibodies » Antibodies-Transcription Factors » Anti -Akt1, phosphorylated (Ser473) (Rac PKa, PKBa)

Anti -Akt1, phosphorylated (Ser473) (Rac PKa, PKBa)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. Akt/PKB phosphorylation is critical for it activity. The major phosphorylation sites required for Akt activation has been identified as threoinine 308 and serine 473. Serine 473 is phosphorylated by MAPKAP kinase 2.
Catalog #A1125-04C
Positive Control PDGF treated NIH 3T3
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No8J226
FormPBS containing 0.2% gelatin and 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenThis monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1. The sequence in this region is identical in human, mouse, chicken, and frog.
SpecificityDetects specifically phosphorylated form of Akt1 expression in PDGF treated cells. Species Crossreactivity: Human, Mouse Predicted to crossreact with Frog and Chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesv-akt Murine Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein Kinase B, RAC, RAC-ALPHA, RAC-alpha Serine/threonine-protein Kinase, RAC-PK-alpha

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