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You are here:Home » Molecular Biology » MB-Protein Kinases » Akt1, active (v-akt Mouse Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) Recombinant

Akt1, active (v-akt Mouse Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA,
Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha)
Recombinant

Pricing

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Specifications

The serine/threonine kinase Akt1 has also been described as Rac-PKa or PKBa. Rac protein kinase family members feature a pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus. The PH domain (amino acids 1-106) has been implicated in interactions with other proteins such as G-protein beta gamma subunits, as well as phosphoinositides. The kinase domain is located between residues 148 to 411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells. Akt1 is a protein of 480 amino acids and has been observed to show different migratory patterns on a western blot according to the state of phosphorylation of the protein. Phosphatase treatment has been shown to result in inactivation of the protein.
Catalog #A1125-06
Recombinant full-length human Akt1, containing N-terminal His6 tag. Expressed by baculovirus in Sf21 cells.
Specific Activity As reported.
Unit Definition One unit of Akt1/PKBa activity is equal to 1nmol phosphate incorporated into 30uM modified Crosstide (GRPRTSSFAEGKK) per minute at 30ºC with a final ATP concentration of 100uM.
Protein Kinase Assay 50-500ng of this enzyme phosphorylates the Akt/SGK substrate peptide (RPRAATF). Akt1/PKBa may also be used to phosphorylate GSK3a (Ser21), GSK3b (Ser9), BAD (Ser136), 6-phosphofructo 2-kinase and Crosstide.
Immunoprecipitation Kinase Assay 500ng of the recombinant Akt1/PKBa may be used as a control for IPK activity.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 12 months.
Molecular Weight59.9kD
SourceRecombinant human
Purity 85% (SDS-PAGE). Activated with GST-MAPKAP-K2 and PDK1. Repurified using Ni2+-NTA agarose..
FormSupplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 150mM imidazole, 0.07% 2-mercaptoethanol, 0.1mM EGTA, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.03% Brij-35
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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