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Akt1, Recombinant, Human (Rac PKa, PKBa)
Akt1, Recombinant, Human (Rac PKa, PKBa)
Pricing
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| Recombinant Human AKT1 produced in SF9 is a glycosylated, polypeptide chain containing 481 amino acids and having a molecular mass of 60kD. AKT1 is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with a N-terminal His-tag. | | | Catalog # | A1125-06H | | Unit Definition | 250 units/mg. One unit of Akt1 activity is equal to 1 nanomole of phosphate transferred to crosstide substrate per minute at 30°C with a final ATP concentration of 100µM. Please note: Kinase activity may vary depending on the substrate and reaction conditions. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 60kD | | Source | Baculovirus, SF9 insect cells | | Purity | 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination. | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in 50mM Tris, pH 7.5, 0.15M sodium chloride, 0.27M sucrose, 10mM beta-mercaptoethanol, 1mM EGTA. Carrier-free | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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