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You are here:Home » Molecular Biology » MB-Protein Kinases » Akt1, Recombinant, Human (Rac PKa, PKBa)

Akt1, Recombinant, Human (Rac PKa, PKBa)

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Specifications

Recombinant Human AKT1 produced in SF9 is a glycosylated, polypeptide chain containing 481 amino acids and having a molecular mass of 60kD. AKT1 is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with a N-terminal His-tag.
Catalog #A1125-06H
Unit Definition250 units/mg. One unit of Akt1 activity is equal to 1 nanomole of phosphate transferred to crosstide substrate per minute at 30°C with a final ATP concentration of 100µM. Please note: Kinase activity may vary depending on the substrate and reaction conditions.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight60kD
SourceBaculovirus, SF9 insect cells
Purity95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination.
Concentration~0.1mg/ml
FormSupplied as a liquid in 50mM Tris, pH 7.5, 0.15M sodium chloride, 0.27M sucrose, 10mM beta-mercaptoethanol, 1mM EGTA. Carrier-free
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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