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You are here:Home » Molecular Biology » MB-Protein Kinases » Akt1, active, Recombinant, Human (RAC-alpha Serine/Threonine-protein Kinase, Protein Kinase B, PKB, Protein Kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha)

Akt1, active, Recombinant, Human (RAC-alpha Serine/Threonine-protein Kinase, Protein
Kinase B, PKB, Protein Kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha)

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Specifications

Akt1 or Protein Kinase B alpha (PKBa) is a serine/threonine kinase that belongs to the Akt family. Akt1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt1 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2) and phosphoinositide-dependent kinase-2 (possibly ILK) (3). Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad (4). In addition, the cyclin dependent kinase inhibitors are substrates of Akt which when phosphorylated relinquish their inhibitory influence on cell cycle progression. Akt also mediates many of the stimulatory effects of insulin on glucose metabolism through deactivation of glycogen synthase kinase, activation of phosphofructo-kinase, and modulation of glucose transporter activity. Akt1 has been shown to play a role in human cancers (4). It is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (5). Akt1 plays an important role in cancer cell survival and proliferation thereby contributing to cancer progression.
Catalog #A1125-06K
SourceRecombinant corresponding to full length human Akt1 containing N-terminal GST tag, expressed by baculovirus in Sf9 insect cells.
Molecular Weight~85kD
Biological Activity124nmol phosphate incorporated into Akt substrate peptide/min/mg protein at 30°C for 15 minutes using a final concentration of 50uM ATP and total of 0.83uCi/ul P-32.
Specific Activity124nmol/min/mg
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight~85kD
SourceSf9 insect cells
Purity~90% (SDS-PAGE)
ConcentrationAs reported
FormSupplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=2.7.11.1


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