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Anti -AKT1, phosphorylated (Tyr326) (RAC-alpha Serine/Threonine-protein Kinase, RAC-PK-alpha, Protein Kinase B, PKB, Proto-oncogene c-Akt, PKB, RAC)
Anti -AKT1, phosphorylated (Tyr326) (RAC-alpha Serine/Threonine-protein Kinase,
RAC-PK-alpha, Protein Kinase B, PKB, Proto-oncogene c-Akt, PKB, RAC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. | | | Catalog # | A1125-11D | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:1,000 | | Western Blot: 1:100-1:500 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in PBS, 0.09% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr326 of human AKT (KLH). | | Specificity | Recognizes human AKT when phosphorylated at Tyr326. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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