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You are here:Home » Antibodies » Antibodies-Protein Kinases » Anti -AKT1, phosphorylated (Ser473) (RAC-alpha Serine/Threonine-protein Kinase, RAC-PK-alpha, Protein Kinase B, PKB, Proto-oncogene c-Akt, PKB, RAC)

Anti -AKT1, phosphorylated (Ser473) (RAC-alpha Serine/Threonine-protein Kinase,
RAC-PK-alpha, Protein Kinase B, PKB, Proto-oncogene c-Akt, PKB, RAC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Catalog #A1125-11E
ApplicationsSuitable for use in ELISA, Western Blot and Dot Blot. Other applications not tested.
Recommended DilutionDot Blot: 1:500
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated synthetic phosphopeptide mapping to a fragment of residues surrounding S473 of human AKT1, UniProt Accession #P31749.
SpecificityRecognizes human AKT1 when phosphorylated at Ser473.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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