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You are here:Home » Antibodies » Antibodies-Protein Kinases » Anti -AKT1 (RAC-alpha Serine/Threonine-protein Kinase, RAC-PK-alpha, Protein Kinase B, PKB, Proto-oncogene c-Akt, PKB, RAC)

Anti -AKT1 (RAC-alpha Serine/Threonine-protein Kinase, RAC-PK-alpha, Protein Kinase B,
PKB, Proto-oncogene c-Akt, PKB, RAC)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Akt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3, which is inactivated by this phosphorylation, the FoxO family of transcription factors, PRAS40, AS160.
Catalog #A1125-11W
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 20ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa surrounding threonine 308 of from human AKT (KLH).
SpecificityRecognizes human AKT1. Species sequence homology: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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