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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -Akt, phosphorylated (Ser505) (PKB, Rac)

Anti -Akt, phosphorylated (Ser505) (PKB, Rac)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC
Akt, also referred to as PKB or Rac, plays a critical role in controlling the balance between survival and apoptosis (13). This protein kinase is activated by insulin and various growth and survival factors, and functions in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and also by phosphorylation within the carboxy-terminus at Ser473. Akt functions to promote cell survival by inhibiting apoptosis by means of its ability to phosphorylate and inactivate several targets, including Bad (5), Forkhead transcription factors (6) and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (7). LY294002 is a specific PI3 kinase inhibitor (8). One of the essential functions of Akt is the regulation of glycogen synthesis through phosphorylation and inactivation of glycogen synthase kinase-3alpha and beta (9,10). Akt may also play a role in insulin stimulation of glucose transport (9). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK3beta-mediated phosphorylation and degredation of cyclin D1 (11), and by negatively regulating the cyclin-dependent kinase inhibitors p27 KIP (12) and p21 WAF1 (13). Akt also plays a critical role in cell growth by directly phosphorylating the mammalian target of rapamycin, mTOR (14), but more importantly through phosophorylation and inactivation of Tuberin (TSC2), an mTOR inhibitor (15). Drosophila Akt (D-Akt) regulates multiple biological
processes such as cell survival (16) and cell growth (17). Major sites of phosphorylation include Ser505 and Thr342. These activation sites are homologous to mammalian
Ser473 and Thr308 respectively. Identified downstream targets of D-Akt include Trh (Ser665) (18) and Tsc2 (19). Like Akt, D-Akt functions in a wortmannin- sensitive pathway involving PI3 kinase (20).
Catalog #A1125-12A
ApplicationsSuitable for use in Western Blotting, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunocytochemistry (IF) 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceDrosophila
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol
PurityProtein A and peptide affinity chromatography
ImmunogenSynthetic phospho peptide (KLH coupled) corresponding to residues around ser505 of drosophila Akt
SpecificityRecognizes endogenous levels of Akt only when phosphorylated at Ser505. Does not recognize drosophila Akt when phosphorylated at other sites, not does it recognize related kinases such as PKC or p70 S6 Kinase
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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