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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -Akt1, (Rac PKa, PKBa), phosphorylated (Thr308)

Anti -Akt1, (Rac PKa, PKBa), phosphorylated (Thr308)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Serum B
Akt1/PKBa is a Ser/Thr protein kinase, whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth. Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3, 4). Three mammalian Akt1/PKBa isoforms have been identified.
Catalog #A1125-19B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:5,000 -10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E459
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySerum
ImmunogenA phospho specific peptide corresponding to residues surrounding Threonine 308 of human Akt1.
SpecificityRecognizes Akt1 phosphorylated at Threonine 308. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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