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Anti -Akt1, (Rac PKa, PKBa), phosphorylated (Thr308)
Anti -Akt1, (Rac PKa, PKBa), phosphorylated (Thr308)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Serum |
B |
|
| Akt1/PKBa is a Ser/Thr protein kinase, whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth. Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3, 4). Three mammalian Akt1/PKBa isoforms have been identified. | | | Catalog # | A1125-19B | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:5,000 -10,000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9E459 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. | | Purity | Serum | | Immunogen | A phospho specific peptide corresponding to residues surrounding Threonine 308 of human Akt1. | | Specificity | Recognizes Akt1 phosphorylated at Threonine 308. Species Crossreactivity: mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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