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You are here:Home » Molecular Biology » MB-Protein Kinases » Akt2, active, Recombinant, Human (RAC-beta Serine/Threonine-protein Kinase, RAC-PK-beta, Protein Kinase Akt-2, Protein Kinase B beta, PKB beta)

Akt2, active, Recombinant, Human (RAC-beta Serine/Threonine-protein Kinase, RAC-PK-beta,
Protein Kinase Akt-2, Protein Kinase B beta, PKB beta)

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Specifications

Akt2 or Protein Kinase B b (PKBb) is a serine/threonine kinase that is a member of the Akt family. Akt2 like the other Akt members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility. The PI3K generates phosphatidylinositol-3, 4, 5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) and phosphoinositide-dependent kinase-2. Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad.
Catalog #A1125-26G
SourceRecombinant corresponding to full-length human Akt2 containing N-terminal His tag, expressed by baculovirus in Sf9 insect cells.
Molecular Weight~58kD
Specific Activity~46nmol phosphate incorporated into Akt/SGK substrate (RPRAATF) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50uM ATP and total of 0.83uCi/ul P-32.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight~58kD
SourceSf9 insect cells
Purity~90% (SDS-PAGE)
ConcentrationAs reported
FormSupplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=2.7.11.1


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