Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E IH

Catalog #	A1206-05
Applications	Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: 1:5000-1:15,000
	Immunohistochemistry: 1:7500. D-Alanine immunoreactive cell bodies located in the lateral thalamus of rat brain. Also, Immunoreactive cell bodies containing D-Alanine in the ventro-lateral thalamus of rat brain.
Specificity	Using a conjugate D-Alanine-Glutaraldehyde-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
	Compound Cross-reactivity ratio (a)
	D-Alanine-G-BSA 1L-Alanine-G-BSA 1/> 50,000Glycine-G-BSA 1/> 50,000D-Valine-G-BSA 1/> 50,000D-Leucine-G-BSA 1/> 50,000
(a)	D-Alanine-G-BSA concentration/unconjugated or conjugated aminoacids concentration at half displacement.
	G = Glutaraldehyde.
	Recommended dilutionThe anti-conjugated b-alanine antibodies can be diluted between 1/2,000-1/10,000.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2. No preservative added.
Purity	Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Immunogen	Synthetic D-Alanine conjugated to BSA.
Specificity	Recognizes protein conjugated D-Alanine. See additional crossreactivity data.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.