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You are here:Home » Antibodies » Abs to Enzymes, Synthetase » Anti -ALAS1 (Aminolevulinate delta-Synthase 1, 5-Aminolevulinic Acid Synthase, Delta-ALA Synthetase)

Anti -ALAS1 (Aminolevulinate delta-Synthase 1, 5-Aminolevulinic Acid Synthase, Delta-ALA
Synthetase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
ALAS-1 (EC 2.3.1.37) is a nuclear-encoded mitochondrial matrix enzyme catalyzing the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependent housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.
Catalog #A1210-02D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHepG2
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa480-530 of human ALAS-1.
SpecificityRecognizes human ALAS1. Species sequence homology: mouse, rat, chimpanzee, canine, pufferfish, bovine, xenopus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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