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You are here:Home » Kits and Assays » Kits-ELISA, BioAssay™ » Albumin, Mouse, BioAssay™ ELISA Kit

Albumin, Mouse, BioAssay™ ELISA Kit


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Catalog #A1274-90T
Intended UseThe Mouse Albumin ELISA Kit is an in vitro immunoassay for the quantification of albumin circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.
Test PrincipleThe Mouse Albumin ELISA kit is based on the binding of mouse albumin in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of albumin present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of albumin in samples and control is calculated from a curve of standards containing known concentrations of albumin.
Kit ComponentsA1274-90T1: Microtiter Plate: 1x96 wells
A1274-90T2: Control (98-182ng/ml), 1x650ul
A1274-90T3: Standard (20ng/ml), 1x650ul
A1274-90T4: Standard (50ng/ml), 1x650ul
A1274-90T5: Standard (100ng/ml), 1x650ul
A1274-90T6: Standard (200ng/ml), 1x650ul
A1274-90T7: Standard (400ng/ml), 1x650ul
A1274-90T8: HRP Conjugate (100X), 1x150ul
A1274-90T9: Sample Diluent (20X), 1x10ml
A1274-90T10: Wash Solution (100X), 1x10ml
A1274-90T11: TMB Substrate, 1x12ml
A1274-90T12: Stop Solution, 1x12ml
Storage and StabilityStore all components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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