| Albumin is synthesized in the liver and consists of a single polypeptide chain with a molecular weight of 66kD. It is present in all body fluids and is most abundant plasma protein. It acts as a transport protein for long chain fatty acids, prostaglandins, hematin, thyroxin, copper II, cystine and pyridoxal phosphate amongst many other substances that bind more weakly. The average serum concentration of albumin is 42g/L. |
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| Catalog # | A1275-01 |
| Applications | Suitable for use in ELISA. Other applications have not been tested. |
| Recommended Dilution | ELISA: 1:12,800-1:25,600. To give an A450nm=1.0 use wells coated with 1:2000 human serum and TMB as a substrate |
| The addition of Tween 20 (0.05-0.1%) is recommended to prevent non-specific binding. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Sheep |
| Source | Human |
| Concentration | ~5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.05% Proclin™ 300, 0.002% methylisothiazolone, 0.002% bromonitrodioxane. Labeled with horseradish peroxidase (HRP). |
| Purity | Purified |
| Immunogen | Human serum albumin purified from Cohn Fraction V. |
| Specificity | Recognizes human serum albumin. Single arc when tested by IEP against human serum. Identity has been confirmed by double diffusion against human serum and a known anti-human albumin. Species Crossreactivity: feline, chicken, bovine, canine, guinea pig, equine, human, mouse, porcine, rabbit and rat. Does not crossreact with goat albumin. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
