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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E B IH Gel

Catalog #	A1275-02
Applications	Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:2000-1:10,000
	ELISA: 1:20,000-1:80,000
	Immunohistochemistry: 1:500-1:2500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Bovine
Concentration	~10mg/ml (UV280)
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml polyethlene glycol
(PEG-8000), 0.01% gentamicin sulfate. Labeled with Horseradish peroxidase (HRP).
Purity	Purified by delipidation, salt fractionation, ion exchange chromatography and dialysis.
Immunogen	BSA
Specificity	Recognizes BSA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified bovine serum albumin. Crossreactivity against albumin from other tissues and species may occur.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.