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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Human serum albumin (HSA) consists of a single polypeptide chain of 584 aminoacids, stabilized by 17 disulfide bridges. HSA is the most common protein in serum, it is produced in the liver and the concentration in serum is 35-50mg/ml. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. The molecular weight is 67.5kD (1).
Catalog #	A1275-28
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:112,000
	Western Blot: After SDS-PAGE, this antibody reacts with native and denatured HSA in non-reduced form only.
	Optimal dilutions to be determined by the researcher.
Culture Medium	RPMI 1640 with 10% fetal calf serum.
Hybridoma	X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F11
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, and15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A/G affinity chromatography from culture supernatant.
Immunogen	Native HSA isolated from human plasma.
Specificity	Recognizes native and denatured HSA. Strong reaction is seen in ELISA with native or denatured HSA directly coated onto the microtiter well, and also in sandwich ELISA in combination with a polyclonal antibody against HSA. Epitope specificity differs from those of A1275-28A and A1275-28B but overlaps as determined by inhibition ELISA.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.