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You are here:Home » Antibodies » Abs to Albumin » Anti -Albumin, Human Serum (HSA)

Anti -Albumin, Human Serum (HSA)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Human serum albumin (HSA) consists of a single polypeptide chain of 584 aminoacids, stabilized by 17 disulfide bridges. HSA is the most common protein in serum, it is produced in the liver and the concentration in serum is 35-50mg/ml. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. The molecular weight is 67.5kD (1).
Catalog #A1275-28A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:8000
Optimal dilutions to be determined by the researcher.
HybridomaX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F12
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, and 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenNative HSA isolated from human plasma.
SpecificityHas specificity for native human serum albumin (HSA). Reacts strongly with native HSA. Strong reaction is seen in ELISA with native HSA directly coated onto the microtiterwell, also when tested in catching ELISA in combination with a polyclonal antibody against HSA. In Western blot after SDS-PAGE, this antibody reacts with native HSA in nonreduced form only. Epitope specificity differs from clones A1275-28 and A1275-28B but overlaps as determined by inhibition ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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