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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Human serum albumin (HSA) consists of a single polypeptide chain of 584 aminoacids, stabilized by 17 disulfide bridges. HSA is the most common protein in serum, it is produced in the liver and the concentration in serum is 35-50mg/ml. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. The molecular weight is 67.5kD (1).
Catalog #	A1275-28B
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:5000
	Optimal dilutions to be determined by the researcher.
Hybridoma	X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F13
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, and15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A/G affinity chromatography from culture supernatant.
Immunogen	Denatured HSA isolated from human plasma.
Specificity	Has specificity for native and denatured human serum albumin (HSA). Reacts strongly with native and denatured HSA. Strong reaction is seen in ELISA with native or denatured HSA directly coated onto the microtiterwell. In Western blotting after SDS-PAGE, this antibody reacts with native and denatured HSA in non-reduced form only. Epitope specificity differs from those of clones 6F11 and 6F12 but overlaps as determined by inhibition ELISA.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.