Albumin is synthesized in the liver and consists of a single polypeptide chain with a molecular weight of 66kD. It is present in all body fluids and is the most abundant plasma protein. It acts as a transport protein for long chain fatty acids, prostaglandins, hematin, thyroxin, copper II, cystine and pyridoxal phosphate amongst many other substances that bind more weakly. The average serum concentration of albumin is 42g/L.
Applications
Suitable for ELISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with murine Balb/c spleen cells.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human Serum Albumin (HSA).
Form
Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins and no preservative added. Also available with sodium azide. See A1275.
Purity
Purified by Protein A affinity chromatography from ascites
Specificity
Recognizes human serum albumin. Does not crossreact with other human proteins, BSA or egg white albumin.