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You are here:Home » Antibodies » Abs to Enzymes, Dehydrogenase » Anti -Alcohol Dehydrogenase, pan (ADH)

Anti -Alcohol Dehydrogenase, pan (ADH)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Highly Purified E B
Catalog #A1330-07
ADH consists of a dimer of identical or nonidentical chains of three types; alpha, beta and gamma. There are 7 different ADH's isozymes in human three belongs to class I: alpha, beta, and gamma, one to class II: pi, one to class III: chi, one to class IV: ADH7 and one to class V: ADH6.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:16,000
Western Blot: 0.3-1.5ug/ml. Detects a band of approximately 38kD (predicted molecular weight: 40kD). ECL. Primary incubation 1 hour.
Optimal dilutions to be determined by the researcher.
Positive ControlHuman liver lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, TBS, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide: STAGKVMKCKA from the N Terminus of the protein sequence for Alcohol Dehydrogenase.
SpecificityReacts with human Alcohol Dehydrogenase. Predicted to react with mouse (90% identity with immunogen), rat (90% identity with immunogen), chimpanzee (90% identity with immunogen) and equine (90% identity with immunogen) due to sequence homology and many other species. Expected to recognize the alpha, beta and gamma polypeptide variants of human alcohol dehydrogenase.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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