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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Alcohol Dehydrogenase BioAssay™ Kit

Alcohol Dehydrogenase BioAssay™ Kit

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Specifications

Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase
enzymes that occur in many organisms and facilitate the interconversion between alcohols
and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other
animals, they serve to break down alcohols which could otherwise be toxic; in yeast and
many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation. The Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (lambda = 450nm). This assay detects ADH activity as low as 0.01mU in samples.
Catalog #A1330-10
Kit ComponentsADH *Assay Buffer 1x25ml
Isopropanol 1x1ml
Developer (Lyophilized) 1x1vial
ADH Positive Control (Lyophilized) 1x1vial
NADH Standard (0.5umol, Lyophilized) 1x1vial.
Storage and StabilityStore the kit at -20°C, protect from light. Allow *Assay Buffer to warm to RT before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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