| Aldosterone is a potent mineral corticoid whose synthesis and release are controlled by the renin-angiotensin system of the body. Aldosterone promotes the re-absorption of sodium in the distal tubules of the kidney resulting in potassium secretion along with sodium retention, which controls the circulating blood volume. Chronic overproduction and secretion of aldosterone leads to hypertension. Measurement of aldosterone levels in serum in conjunction with plasma renin levels can be used to differentiate between primary and secondary aldosteronism. |
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| Catalog # | A1350-05 |
| Test Principle | Aldosterone ELISA kit is based upon competitive solid phase ELISA. The sample competes with Streptavidin-Biotin-linked Aldosterone for a fixed and limited number of antibody binding sites. In the assay, the Aldosterone standard or samples serum are incubated with Aldosterone-HRP conjugate and anti-Aldosterone. In this solid-phase system, the antibody bound Aldosterone will remain on the well while unbound Aldosterone will be removed by washing. A color (blue) is developed when the substrate, TMB is mixed with the antibody bound Aldosterone-HRP conjugate. After a short incubation, the enzyme reaction is stopped (blue color turns yellow) and the intensity of the color (yellow) is measured using an ELISA plate reader. The color is inversely proportional to the concentration of Aldosterone in the sample. |
| Sample Type | Serum. Aldosterone kit has been used in mouse/rat serum and plasma (see published references). Other fluids may be adapted. |
| Intended Use | For quantitative determination of Aldosterone in human serum. |
| Sensitivity | ~15pg/ml |
| Range | Unrestricted salt intake, seated position |
| N=54 Mean = 105pg/ml |
| Expected range: 25-315pg/ml. |
| Unrestricted salt intake Urine (5-19ug/24 hr). |
| Kit Components | A1350-05A: Microwell Strip Plate 1x96 wells. Coated with Anti-Aldosterone IgG |
| A1350-05B: Standard 0pg/ml 1x10ml Ready to use |
| A1350-05C: Standard 20pg/ml 1x500ul Ready to use |
| A1350-05D: Standard 80pg/ml 1x500ul Ready to use |
| A1350-05E: Standard 300pg/ml 1x500ul Ready to use |
| A1350-05F: Standard 800pg/ml 1x500ul Ready to use |
| A1350-05G: Standard 2000pg/ml 1x500ul Ready to use |
| A1350-05H: Aldosterone Control 1x500ul Ready to use |
| A1350-05J: Aldosterone-Avidin (HRP) 50x 1x100ul |
| A1350-05K: Assay Buffer 1x15ml Ready to use |
| A1350-05L: Wash Buffer, 10x 1x50ml |
| A1350-05M: Substrate (TMB) Solution 1x16ml |
| A1350-05N: Stop Solution 1x6ml |
| Storage and Stability | Aliquot and store Standards and Control at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
