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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Alkaline Phosphatase, Bovine (AP)

Alkaline Phosphatase, Bovine (AP)

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Specifications

Alkaline Phosphatase is Dimeric protein having a molecular weight of 140kD, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity. Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Catalog #A1355-65B
Activity~1000U/mg (pH 9.6, 37°C, with 0.025M glycine, 10% glycerol as buffer).
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight140kD
SourceCalf Intestine
Purity95% pure by Gel Filtration.
ConcentrationAs reported
FormSupplied as a liquid in 5mM MgCl2, 0.1mM ZnCl2, 5mM TRIS, pH 7.0, 50% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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