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You are here:Home » Molecular Biology » MB-Enzymes » ALKBH2, Recombinant, Human, aa1-261, His-tag (ABH2, Oxy DC1)

ALKBH2, Recombinant, Human, aa1-261, His-tag (ABH2, Oxy DC1)

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Specifications

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, also known ALKBH2, belongs to the ALKB family. Expressed in heart, colon, liver, testis, ovary, prostate and small intestine, ALKBH2 uses iron as a cofactor and functions as a dioxygenase that catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and a-ketoglutarate for enzymatic activity.
Catalog #A1355-82
SourceRecombinant corresponding to aa1-261 from human ALKBH2, fused to His-tag at N-terminus expressed in E.coli.
AA SequenceMGSSHHHHHH SSGLVPRGSH MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR
YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K
Molecular Weight~31.4kD (281aa), confirmed by MALDI-TOF
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~31.4kD
SourceE. coli
Purity~90% (SDS-PAGE)
Concentration~0.5mg/ml
FormSupplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 1mM DTT, 30% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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