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You are here:Home » Molecular Biology » MB-Enzymes, Oxidase » ALKBH3, Recombinant, Human, aa1-286, His-tag (Alpha-ketoglutarate-dependent Dioxygenase alkB Homolog 3, Alkylated DNA Repair Protein alkB Homolog 3, Prostate Cancer Antigen 1, DEPC-1, DEPC1, ABH3)

ALKBH3, Recombinant, Human, aa1-286, His-tag (Alpha-ketoglutarate-dependent Dioxygenase
alkB Homolog 3, Alkylated DNA Repair Protein alkB Homolog 3, Prostate Cancer Antigen 1,
DEPC-1, DEPC1, ABH3)

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Specifications

ALKBH3 is a member of the ALKB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates. This protein repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA and its activity is stimulated by ascorbate. ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus.
Catalog #A1355-83C
SourceRecombinant corresponding to aa1-286 from human ALKBH3, fused to His-tag at N-terminus expressed in E.coli.
AA SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELDM EEKRRRARVQ GAWAAPVKSQ AIAQPATTAK SHLHQKPGQT WKNKEHHLSD REFVFKEPQQ VVRRAPEPRV IDREGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDITYQQPR LTAWYGELPY TYSRITMEPN PHWHPVLRTL KNRIEENTGH TFNSLLCNLY RNEKDSVDWH SDDEPSLGRC PIIASLSFGA TRTFEMRKKP PPEENGDYTY VERVKIPLDH GTLLIMEGAT QADWQHRVPK EYHSREPRVN LTFRTVYPDP RGAPW
Molecular Weight~37.9kD (325aa), confirmed by MALDI-TOF
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~37.9kD
SourceE.coli
Purity~95% (SDS-PAGE)
Concentration~1mg/ml
FormSupplied as a liquid in 20mM Tris-HCl buffer, pH 8, 0.1M sodium chloride, 2mM DTT, 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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