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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Allograft Inflammatory Factor-1 (AIF-1, IBA1, IRT-1)

Anti -Allograft Inflammatory Factor-1 (AIF-1, IBA1, IRT-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Allograft-inflammatory factor-1 (AIF-1) polypeptide, also known as Ionized calcium-binding adapter molecule 1, Iba-1. AIF-1 is a 17kD IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis.
Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region.
Catalog #A1358-56
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Paraffin): 1:50. Antigen retrieval is recommended using a combination of microwave and a sodium citrate solution.
Western Blot: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No6A479
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman Allograft Inflammatory Factor-1
SpecificityRecognizes human Allograft Inflammatory Factor-1. Species Crossreactivity: rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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