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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » AluI

AluI

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Specifications

5'-A G^C T-3'
3'-T C^G A-5'
Catalog #A1365
Concentration 10u/ul
Source Arthrobacter luteus
Buffer 33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37°C.
Diluent Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer.
Storage Buffer 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with AluI.
Ligation/Recutting AssayAfter 50-fold overdigestion (3u/ug DNA x 17 hours) with AluI approximately 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.4uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Methylation EffectsBlocked by overlapping EcoBI methylation.
Stability during Prolonged IncubationA minimum of 0.1units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Thermal Inactivation Enzyme is inactivated by incubation at 65°C for 20min.
Number of Recognition Sites in DNA
Lambda143
PhiX17424
M13mp18/1927
pBR32217
pUC18/1916
pUC5716
pTZ19R/U17
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.


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