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Anti -AMIGO3 (Amphoterin-Induced Gene and ORF 3, Alivin-3)
Anti -AMIGO3 (Amphoterin-Induced Gene and ORF 3, Alivin-3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Purified |
E B IH |
|
| AMIGO3 (Amphoterin-Induced Gene and ORF 3) is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (CRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied. AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and 2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with that of rat and human AMIGO3, respectively. | | | Catalog # | A1372-59 | | Applications | Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Direct ELISA: 0.5- 1.0ug/ml detection limit for rmAMIGO3 is ~ 1ng/well. | | Western Blot: 1-2ug/ml detection limit for rmAMIGO3 is ~ 25ng/lane under non-reducing and reducing conditions. | | Immunohistochemistry: 25ug/ml. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 8H161 | | Host | Rat | | Source | Mouse | | Concentration | As reported | | Form | Supplied as a lyophilized powder in 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | NS0-derived rmAMIGO3. (aa 20 - 378) | | Specificity | Recognizes mouse AMIGO3. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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