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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -AMIGO3 (Amphoterin-Induced Gene and ORF 3, Alivin-3)

Anti -AMIGO3 (Amphoterin-Induced Gene and ORF 3, Alivin-3)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified E B IH
AMIGO3 (Amphoterin-Induced Gene and ORF 3) is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (CRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied. AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and 2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with that of rat and human AMIGO3, respectively.
Catalog #A1372-59
ApplicationsSuitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5- 1.0ug/ml detection limit for rmAMIGO3 is ~ 1ng/well.
Western Blot: 1-2ug/ml detection limit for rmAMIGO3 is ~ 25ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 25ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No8H161
HostRat
SourceMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder in 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived rmAMIGO3. (aa 20 - 378)
SpecificityRecognizes mouse AMIGO3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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