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You are here:Home » Molecular Biology » MB-Apoptosis » 7-Amino-4-trifluoromethylcoumarin (AFC)

7-Amino-4-trifluoromethylcoumarin (AFC)


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Catalog #A1381-50
SolubilitySoluble in methanol or 25mg/ml DMSO.
ApplicationsThe free form of AFC can be used as a fluorescent marker and standard in fluororometric caspase assays.
ProtocolPrepare a 1mM solutionof AFC in methanol or DMSO and then dilute it into aqueous buffer at different concentrations within the 0-4uM range. Read 100ul/sample.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with methanol or DMSO. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular FormulaC10H6NO2F3
Molecular Weight229.2
Purity> 99% (TLC)
FormSupplied as a yellow powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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