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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -AML-1, phosphorylated (Ser249)

Anti -AML-1, phosphorylated (Ser249)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP FC IF
AML1 (also known as Runx1, CBFA2 and PEBP2aB) is a member of the CBF (core binding factor) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3,4,5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFb and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21)TEL-AML and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an ERK-dependent manner (7,8)
Catalog #A1383-01
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern blot 1:1000
Immunoprecipitation 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide (KLH-coupled) corresponding to amino acids around Ser249 of human AML1.
SpecificityDetects endogenous levels of AML1 protein only when phosphorylated on Ser249. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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