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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP FC IF

AML1 (also known as Runx1, CBFA2 and PEBP2aB) is a member of the CBF (core binding factor) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3,4,5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFb and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21)TEL-AML and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an ERK-dependent manner (7,8)
Catalog #	A1383-01
Applications	Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western blot 1:1000
	Immunoprecipitation 1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide (KLH-coupled) corresponding to amino acids around Ser249 of human AML1.
Specificity	Detects endogenous levels of AML1 protein only when phosphorylated on Ser249. Species Crossreactivity: Human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.