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Anti -AMP Activated Protein Kinase alpha1 (AMPK a1)
Anti -AMP Activated Protein Kinase alpha1 (AMPK a1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH |
|
| The 5’-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non–fermentor) kinase family (1) is a heterotrimeric protein comprising alpha (63kD), beta (38kD) and gamma (38kD) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non–catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl- CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. | | | Catalog # | A1475-01H | | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:5000 | | Immunohistochemistry: 1:100-1:250 | | Immunoprecipitation: 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 6k21 | | Host | Rabbit | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a liquid in 50mM Tri-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol. | | Purity | Supernatant | | Immunogen | A synthetic peptide corresponding to residues near the C-term of human AMPK alpha-1 subunit. | | Specificity | Recognizes human AMPK alpha-1 subunit. Species Crossreactivity: mouse and rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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