| AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit, which is stabilised by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia, ischemia, exercise, low glucose levels. |
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| AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. Recent studies suggest that AMPK functions as a suppressor of cell proliferation of both malignant and normal cells. |
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| Catalog # | A1475-01P4 |
| Applications | Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:100-1:1,000. Detects a band of ~63kD in rat brain cell lysates. |
| Immunohistochemistry: Frozen sections |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 10B156 |
| Host | Mouse |
| Source | Drosophila |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to 29aa within the N-terminal catalytic domain of Drosophila AMPK alpha. Species Sequence Homology: Vertebrates, Human, Mouse AND Rat |
| Specificity | Recognizes both the alpha 1 and the alpha 2 isoforms of Drosophila AMP-activated protein kinase (AMPK). Species sequence homology: vertebrates, human, mouse, rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | 5 AMP activated protein kinase catalytic alpha 1 chain, 5' AMP activated protein kinase catalytic subunit alpha 1, AAPK1, AMPK alpha 1 chain, AMPK, AMPK1, AMPKa1, HMG CoA reductase kinase, MGC33776, MGC57364, PRKAA 1, PRKAA1, Protein kinase AMP activated |
