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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMPK1 (Ser487) (5'-AMP-activated Protein Kinase, Catalytic Subunit alpha-1 Chain, AMPK Subunit alpha-1, PRKAA1)

Anti -AMPK1 (Ser487) (5'-AMP-activated Protein Kinase, Catalytic Subunit alpha-1 Chain,
AMPK Subunit alpha-1, PRKAA1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Catalog #A1475-01Q1
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic non-phosphoeptide corresponding to amino acid residues surrounding Ser487 of human PRKAA1 (KLH).
SpecificityRecognizes human PRKAA1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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