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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMPK1, CT (5'-AMP-activated Protein Kinase, Catalytic Subunit alpha-1 Chain, AMPK Subunit alpha-1, PRKAA1)

Anti -AMPK1, CT (5'-AMP-activated Protein Kinase, Catalytic Subunit alpha-1 Chain, AMPK
Subunit alpha-1, PRKAA1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.
Catalog #A1475-01Q3
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide selected from the C-terminal region of human AMPK alpha 1 (KLH).
SpecificityRecognizes human AMPK alpha 1. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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