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Anti -AMP Activated Protein Kinase alpha2 (AMPK a2)
Anti -AMP Activated Protein Kinase alpha2 (AMPK a2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria, catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, is the rate limiting step of mitochondrial fatty acid ?-oxidation. Activities of ACC-1, ACC-2 and other key proteins of carbohydrate and fat metabolism are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). AMPK switches-off biosynthetic processes when ATP levels are depleted and AMP rises in response to fuel deficiency and treatments like heat shock, ischaemia and exercise. A defect in AMPK switch leads to insulin resistance, dyslipidemia, ketosis resistance and other metabolic derangements in Type 2 diabetes. AMPK also regulates cholesterol biosynthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA resuctase. | | | Catalog # | A1475-02G | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 1-10ug/ml (ECL). | | ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. | | Optimal dilutions to be determined by the researcher. | | Control Peptide | A1475-02H: AMP Activated Protein Kinase alpha2, Rat, Control Peptide (AMPK a2) | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.1% BSA | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to 20aa near the C terminus of rat AMPK-a2, conjugatd to KLH. Species Sequence Homology | | Specificity | Recognizes rat AMPK-a2. No significant sequence homology is seen with AMPK-a1 or other proteins. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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