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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMP Activated Protein Kinase alpha2 (AMPK a2) (Agarose)

Anti -AMP Activated Protein Kinase alpha2 (AMPK a2) (Agarose)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IP
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Catalog #A1475-02N
ApplicationsSuitable for use in Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoprecipitation: 15-25ul gel slurry per 0.1-1mg protein
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
ConcentrationNot determined
FormSupplied as a 50% slurry in 0.2ml agarose gel, with PBS, pH 7.2, and 0.09% sodium azide. AMPK alpha 2 antibody was coupled to agarose beads using a cyanogen bromide method
PurityUnconjugated antibody was purifed by affinity chromatography
ImmunogenSynthetic peptide which represents a portion of AMP-activated protein kinase, alpha-2 catalytic subunit encoded within exon 7 (LocusLink ID 5563).
SpecificityRecognizes human, rat, and bovine AMPK alpha 2
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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