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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMP Activated Protein Kinase beta1 (AMPK b1)

Anti -AMP Activated Protein Kinase beta1 (AMPK b1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals, and plays a key role in regulation of energy homeostasis (1). The kinase is activated by an elevated AMP-ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia (2). AMPK is also involved in apoptosis (3). AMP plays an essential role in the activation of AMPK. Firstly, it is required for AMPK phosphorylation by the upstream kinase AMPKK at Thr172 (4). AMPK phosphorylation by AMPKK raises AMPK
activity at least 50-fold. Secondly, AMP inhibits the dephosphorylation and inactivation of AMPK by PP2C (5). The beta1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation. Phosphorylation at serine 108 of the beta1 subunit seems to be required for the activation of AMPK enzyme. Phosphorylation at serines 24, 25 and 182 of the beta1 subunit affects its cellular localization (6).
Catalog #A1475-03A
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityProtein A and peptide affinity chromatography
ImmunogenSynthetic peptide corresponding to the amino-terminal residues of human AMPKbeta1 (KLH coupled).
SpecificityDetects endogenous levels of total AMPKbeta1. Does not crossreact with AMPK beta2. Species Crossreactivity: human, mouse, rat and monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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