| AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals, and plays a key role in regulation of energy homeostasis (1). The kinase is activated by an elevated AMP-ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia (2). AMPK also has a role in apoptosis (3). AMP plays an essential role in the activation of AMPK. Firstly, it is required for AMPK phosphorylation by the upstream kinase AMPKK at Thr172 (4). AMPK phosphorylation by AMPKK raises AMPK |
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| activity at least 50-fold. Secondly, AMP also inhibits the dephosphorylation and inactivation of AMPK by PP2C (5). The beta1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation. Phosphorylation at serine 108 of the beta1 subunit has |
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| been suggested to enhance the activity of AMPK enzyme (6). Phosphorylation at serines 24, 25 and 182 of the beta1 subunit affects its cellular localization (6). |
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| Catalog # | A1475-03B |
| Applications | Suitable for use in Western Blotting, Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000 |
| Immunoprecipitation: 1:50 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. |
| Purity | Protein A and peptide affinity chromatography. |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding serine 108 of human AMPK beta1. |
| Specificity | Recognizes endogenous levels of human AMPK beta1 at ~38kD only when phosphorylated at serine 108. May crossreact with phosphorylated AMPK beta2 when phosphorylated at Ser109. Species Crossreactivity: Mouse, rat, monkey |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
