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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMP Activated Protein Kinase beta1 (AMPK b1)

Anti -AMP Activated Protein Kinase beta1 (AMPK b1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit B IP IH FC
Catalog #A1475-03K
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50
Western Blot: 1:1000
Immunohistochemistry: 1:100-1:250
Immunoprecipitation:1:80
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quality Control Testing Immunoblot Analysis A 1:1,000 to 1:5,000 dilution of the lot detected endogenous AMPKb1 lysates from NIH3T3, HeLa, A431, and PC12 cells. Immunoprecipitation: A 1:80 dilution of this antibody immunoprecipitated AMPKb1 using protein G:agarose beads. Flow Cytometry: A 1:50 dilution of this antibody stained cells for intracellular staining for flow Cytometry analysis. Immunohistochemistry: A 1:100 to 1:250 dilution of this antibody is used for the immunohistochemical analysis of Paraffin-embedded human lung carcinoma using the anti-AMPKb1 antibody. Immunoblot Analysis: Lysates from NIH3T3, HeLa, A431, and PC12 (lanes 1, 2, 3, and 4, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-AMPKb1 (1:5,000). Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates AMPKb1 (38kD).
Clone TypeMonoclonal
IsotypeIgG
Clone No7i8
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M NaCl, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
ImmunogenSynthetic peptide corresponding to human AMPKb1
SpecificityRecognizes AMPKb1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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