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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMP Activated Protein Kinase beta1 (5'-AMP-activated Protein Kinase Subunit beta-1, AMPK Subunit beta-1, AMPKb, PRKAB1, AMPK)

Anti -AMP Activated Protein Kinase beta1 (5'-AMP-activated Protein Kinase Subunit beta-1,
AMPK Subunit beta-1, AMPKb, PRKAB1, AMPK)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals, and plays a key role in regulation of energy homeostasis. The kinase is activated by an elevated AMP-ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia. AMPK is also involved in apoptosis. AMP plays an essential role in the activation of AMPK. Firstly, it is required for AMPK phosphorylation by the upstream kinase AMPKK at Thr172. AMPK phosphorylation by AMPKK raises AMPK activity at least 50-fold. Secondly, AMP inhibits the dephosphorylation and inactivation of AMPK by PP2C. The beta1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation. Phosphorylation at serine 108 of the beta1 subunit seems to be required for the activation of AMPK enzyme. Phosphorylation at serines 24, 25 and 182 of the beta1 subunit affects its cellular localization.
Catalog #A1475-03M
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:20,000
Western Blot: 1:500-1:1,000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic non-phosphopeptide corresponding to human AMPKbeta1 around the phosphorylation site of serine 182.
SpecificityRecognizes human AMPKb. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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