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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMP Activated Protein Kinase alpha, phosphorylated (Thr172) (AMPKa) (BSA and Azide Free)

Anti -AMP Activated Protein Kinase alpha, phosphorylated (Thr172) (AMPKa) (BSA and Azide
Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals, and it plays a key role in regulation of energy homeostasis. The kinase is activated by an elevated AMP-ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia. AMPK also has a role in apoptosis. AMP plays an essential role in the activation of AMPK. Firstly, it is required for AMPK phosphorylation by the upstream kinase AMPKK at Thr172. AMPK phosphorylation by AMPKK raises AMPK activity at least 50-fold. Secondly, AMP also inhibits the dephosphorylation and inactivation of AMPK by PP2C.
Catalog #A1475-05C
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000 (Incubate membrane with the diluted antibody in 5%BSA, 1XTBS, o.1% Tween-20 at 4ºC with gentle shaking overnight.)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Thr172 of human AMPK-alpha (KLH coupled).
SpecificityDetects endogenous AMPK-alpha only when activated by phosphorylation at Thr172. Detects both alpha-1 and alpha-2 isoforms of the catalytic subunit. It does not detect the regulatory beta or gamma subunits. Species crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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