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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMP Activated Protein Kinase, pan (AMPK)

Anti -AMP Activated Protein Kinase, pan (AMPK)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #A1475-06
ApplicationsSuitable for use in Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot : 0.5-2microgram/ml detected AMP-activated protein kinase a subunits in a rat liver
protein preparation.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 4-22 of human AMPactivated protein a1 catalytic subunit
(AEKQKHDGRVKIGHYILGD). The sequence shares 18/19 residues with rat and human AMP-activated protein kinase a2 catalytic subunit.
SpecificityRecognizes AMP-activated protein kinase a subunits, Mr ~63kD. An unknown protein
was also detected, Mr ~44kD, that was competed with the immunizing peptide. Species CrossreactivityRat and human.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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