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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMP Activated Protein Kinase 1, phosphorylated Ser485 (AMPK)

Anti -AMP Activated Protein Kinase 1, phosphorylated Ser485 (AMPK)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits, each of which is encoded by two or three distinct genes (alpha1, 2; beta 1, 2; gamma1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPK alpha at Thr172 in the activation loop and this phosphorylation is required for AMPK activation (3-5). AMPK alpha is also phosphorylated at Thr258 and Ser485 (for alpha1; Ser491 for alpha2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The beta1 subunit is post-translationally modified by myristoylation and multisite phosphorylation including Ser24/25, Ser96, Ser101, Ser108 and Ser182 (6,7). Phosphorylation at Ser108 of the beta1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPK gamma subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1).
Catalog #A1475-06A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No7H5
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, 150mM sodium chloride 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenImmunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser485 of human AMPK alpha1 (Q13131).
SpecificityRecognizes endogenous levels of human AMPK alpha1 only when phosphorylated at Ser485. The antibody does not cross-react with phosphorylated AMPK alpha2 or other related proteins. Species Crossreactivity: hamster, monkey, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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