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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMPK1, phosphorylated (Thr174) (5'-AMP-activated Protein Kinase Catalytic Subunit alpha-1, AMPK Subunit alpha-1, PRKAA1)

Anti -AMPK1, phosphorylated (Thr174) (5'-AMP-activated Protein Kinase Catalytic Subunit
alpha-1, AMPK Subunit alpha-1, PRKAA1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
AMPK1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime- AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Catalog #A1475-06D
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human AMPK1 around the phosphorylation site of threonine 174 (L-R-TP-S-C).
SpecificityRecognizes human AMPK1 when phosphorylated at Thr174. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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