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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMP Activated Protein Kinase gamma1, CT (AMPK g1)

Anti -AMP Activated Protein Kinase gamma1, CT (AMPK g1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP FC
The 5’-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non–fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising (63kD), (38kD) and (38kD) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non–catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).
Catalog #A1475-07B
ApplicationsSuitable for use in Flow Cytometry, Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:20
Western Blotting: 1:1,000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k22
HostRabbit
SourceHuman
FormSupplied as a liquid
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues in the C-term of human AMPK gamma-1 subunit
SpecificityRecognizes human AMPK gamma-1 subunit
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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