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Anti -AMP Activated Protein Kinase gamma1, NT (AMPK g1)
Anti -AMP Activated Protein Kinase gamma1, NT (AMPK g1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH IC FC |
|
| The 5’-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non–fermentor) kinase family (1), is a heterotrimeric protein comprise of alpha (63kD), beta (30kD) and gamma (38kD) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non–catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxy- methylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3). | | | Catalog # | A1475-07C | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:2000 | | Immunoprecipitation: 1:50 | | Flow Cytometry: 1:100 | | Immunocytochemistry: 1:50-1:100 | | Immunohistochemistry: 1:250-1:500 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 6k23 | | Host | Rabbit | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol. | | Purity | Supernatant | | Immunogen | A synthetic peptide corresponding to residues in the N-term of human AMPK gamma-1 subunits | | Specificity | Recognizes human AMPK gamma-1 subunits at ~38kD. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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