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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AMP Activated Protein Kinase 1, 2, phosphorylated (Ser485/Ser491) (AMPK1, AMPK2)

Anti -AMP Activated Protein Kinase 1, 2, phosphorylated (Ser485/Ser491) (AMPK1, AMPK2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
AMP-activated protein kinase (AMPK) is the downstream component of a protein kinase cascade that acts as an intracellular energy sensor maintaining the energy balance within the cell. This pivotal role of AMPK places it in an ideal position for regulating whole-body energy metabolism. AMPK is activated in response to ATP depletion, which causes a concomitant increase in the AMP:ATP ratio . Once activated, it phosphorylates several downstream substrates, the overall effect of which is to switch off ATP-consuming pathways (e.g. fatty acid synthesis and cholesterol synthesis) and to switch on ATP-generating pathways (e.g. fatty acid oxidation and glycolysis). The protein also plays a part in protecting the body from metabolic diseases such as type 2 diabetes and obesity. Structurally AMPK is a heterotrimeric complex comprising a catalytic subunit (alpha) and two regulatory subunits (beta and gamma).
Catalog #A1475-08E4
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human AMPK1/AMPK2 around the phosphorylation site of serine 485/491 (S-G- SP-V-S).
SpecificityRecognizes human AMPK1/AMPK2, phosphorylated at Ser485/Ser491. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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