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Anti -AMP Activated Protein Kinase 1, 2 (AMPK1, AMPK2)
Anti -AMP Activated Protein Kinase 1, 2 (AMPK1, AMPK2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
IH |
|
| AMP-activated protein kinase (AMPK) is the downstream component of a protein kinase cascade that acts as an intracellular energy sensor maintaining the energy balance within the cell. This pivotal role of AMPK places it in an ideal position for regulating whole-body energy metabolism. AMPK is activated in response to ATP depletion, which causes a concomitant increase in the AMP:ATP ratio . Once activated, it phosphorylates several downstream substrates, the overall effect of which is to switch off ATP-consuming pathways (e.g. fatty acid synthesis and cholesterol synthesis) and to switch on ATP-generating pathways (e.g. fatty acid oxidation and glycolysis). The protein also plays a part in protecting the body from metabolic diseases such as type 2 diabetes and obesity. Structurally AMPK is a heterotrimeric complex comprising a catalytic subunit (alpha) and two regulatory subunits (beta and gamma). | | | Catalog # | A1475-08E5 | | Applications | Suitable for use in Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic non-phosphopeptide corresponding to human AMPK1/AMPK2 around the phosphorylation site of serine 485/491 (S-G-SP-V-S). | | Specificity | Recognizes human AMPK1/AMPK2. Species Crossreactivity: mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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