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You are here:Home » Antibodies » Abs to Disease Markers » Anti -AMPH (AMPH1, amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen), HGNC:471)

Anti -AMPH (AMPH1, amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen),
HGNC:471)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH
Amphiphysin I is a 76kD protein with an apparent molecular mass of 128kD, that associates with the cytoplasmic surface of synaptic vesicle (1). Amphiphysin is highly enriched in nerve terminal, where it has a putative role in clathrin-dependent recycling of secretory vesicle (2). Amphiphysin binds dynamin 1 and synaptojanin 1 through carboxy terminal SH3 domains (3). Additional interactions have been observed with clathrin and AP-2. It is through these interactions that amphiphysin is thought to play a role in clathrin mediated endocytosis. Amphiphysin is the dominant autoantigen in paraneoplastic Stiff-Man syndrome (4).
Catalog #A1580
ApplicationsWestern Blot (Colorimetric): 0.5ug/ml
Immunoprecipitation
Immunohistochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlsRat Brain Tissue Extract
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No4E8
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.1mM PMSF.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human amphiphysin
SpecificityDetects a 128kD protein, corresponding to the apparent molecular mass of amphiphysin I on SDS-PAGE immunoblots, in samples from human, monkey, rat, mouse, rabbit and bovine. A 108kD isoform may be observed in some tissues or cell lysates. The epitope is located within the carboxyl terminal region of amphiphysin I (residues 545695). Rat brain immunostaining using this antibody in immunofluorescence shows synaptic distribution. Species Reactivity: human, monkey, rat, mouse, rabbit, bovine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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