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Anti -AMPH (AMPH1, amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen), HGNC:471)
Anti -AMPH (AMPH1, amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen),
HGNC:471)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH |
|
| Amphiphysin I is a 76kD protein with an apparent molecular mass of 128kD, that associates with the cytoplasmic surface of synaptic vesicle (1). Amphiphysin is highly enriched in nerve terminal, where it has a putative role in clathrin-dependent recycling of secretory vesicle (2). Amphiphysin binds dynamin 1 and synaptojanin 1 through carboxy terminal SH3 domains (3). Additional interactions have been observed with clathrin and AP-2. It is through these interactions that amphiphysin is thought to play a role in clathrin mediated endocytosis. Amphiphysin is the dominant autoantigen in paraneoplastic Stiff-Man syndrome (4). | | | Catalog # | A1580 | | Applications | Western Blot (Colorimetric): 0.5ug/ml | | Immunoprecipitation | | Immunohistochemistry: 5ug/ml | | Optimal dilutions to be determined by the researcher. | | Positive Controls | Rat Brain Tissue Extract | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 4E8 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS, 0.1mM PMSF. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant human amphiphysin | | Specificity | Detects a 128kD protein, corresponding to the apparent molecular mass of amphiphysin I on SDS-PAGE immunoblots, in samples from human, monkey, rat, mouse, rabbit and bovine. A 108kD isoform may be observed in some tissues or cell lysates. The epitope is located within the carboxyl terminal region of amphiphysin I (residues 545–695). Rat brain immunostaining using this antibody in immunofluorescence shows synaptic distribution. Species Reactivity: human, monkey, rat, mouse, rabbit, bovine | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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